Drug Testing Instructions

Once enrolled, only DOT Drug Tests ordered through Express Labs are accepted.  If Online Ordering is unavailable, use the Quest Paper Forms provided by Express Labs.

Online Drug Test Ordering

1. Log in to your Express Labs account and click Order Drug Tests
2. Select the Reason for Testing
3. Use the Site Locator to select a collection site
4. Click Complete Order
5. Call to confirm the site is open and accepting walk-ins
6. Check your email for the QPassport (usually within 1 hour), then bring it with photo ID to the site

Quest Paper Forms

Bring the Quest Paper Forms provided by Express Labs with Photo ID to any Quest Diagnostics collection site.

• In-Network Sites (collection fees included) - View Collection Sites
• Out-of-Network Sites (collection fees not included) - Search Google for "DOT Drug Testing"
• Call to confirm the site is open and accepting walk-ins

Important Reminders

• Drivers should only sign DOT forms listing their employer/company name
• Most sites will not begin a DOT collection within 3 hours of closing
• Members are charged for results, not QPassport orders
• Drivers must carry Quest Paper Forms provided by Express Labs

Drug Test Results

1. Emailed to the Designated Employer Representative (DER)
2. Uploaded to your Express Labs account
3. Available in Real-Time Test Tracking (ESP) - Request Access

• Negative results are typically available within 24-48 hours
• Non-negative results may require additional confirmation, which can take 3-5 business days
• Questions about delayed results?  Contact Quest Diagnostics at 800-877-7484 with your QPassport or Specimen ID

Drug Testing Fees

• DOT & Non-DOT Drug Tests: $80
• Return-to-Duty & Follow-Up Drug Tests (Observed): $125
• Online Ordering: Collection fees included
• Quest Paper Forms: Collection fees included at In-Network sites only
• Out-of-Network Sites: Collection fees not included
• Invoices are due upon receipt.  A valid credit card is required to maintain active accounts

DOT Drug Testing Process

1. Collection Site

• Driver provides a urine specimen at a DOT-certified collection site
• Specimen is sealed and shipped to Quest Diagnostics

2. Laboratory Testing

• Quest Diagnostics performs the required DOT testing
• Non-negative specimens receive confirmation testing

3. Medical Review Officer (MRO)

• Negative results are reported as negative
• The MRO reviews all non-negative results and contacts the driver if necessary

Express Labs FMCSA Random Drug & Alcohol Consortium
Drug Testing | Updated 2026