Drug Testing Instructions

Order DOT drug tests online through your Express Labs account using QPassports.

• If you are unable to order online, use Quest paper forms provided by Express Labs
• All DOT drug tests must be ordered through Express Labs; results from outside consortiums or third-party programs are not accepted.

Option 1: Ordering Drug Tests Online Instructions

1. Login to your Express Labs account
2. Click “Order Drug Tests” on top tabs
3. Select the Reason (pre-employment, random)
4. Site Locator - Enter the ZIP code to locate a collection site
5. Click “Complete Order”
6. Check your email for the QPassport from Quest (usually within 1 hour)
7. Review your order (only charged for results not orders)
8. Drivers must bring the QPassport (printed or on phone) to the site selected
9. Drivers should only sign forms that list their name and employer/company name

• Call the collection site after ordering the test to confirm they are open and accepting walk-ins before reporting for testing or notifying the driver
• Collection fees are included when ordering online

Option 2: Quest Paper Forms Instructions

If you can not order online use the Quest paper forms (provided by Express Labs).  DOT requires drivers to keep Quest paper forms that Express Labs provides on their person in the event of an accident or random selection and you are unable to order online.

1. In-Network Sites - View Sites
2. Out-of-Network Sites: Google "DOT Drug Testing" for Concentra, Urgent Cares
3. Check reason on form (pre-employment, random)
4. Drivers must bring Quest paper form (provided by Express Labs) to the site
5. Drivers should only sign forms that list their name and employer/company name

• Call the Collection Site to confirm they are open and accepting walk-ins after ordering the test to obtain the QPassport before reporting for testing or notifying the driver
• Collection Fees - Included at In-Network sites.  Not included at Out-of-Network sites
• Quest Paper Forms: Shipped upon enrolling.  Order additional forms in your account.  Forms can not be emailed or faxed

Drug Test Results

Drug test results are emailed and uploaded into your account from Express Labs Medical Review Officer (MRO).

• Negative results are usually reported within 3 business days
• After 4 business days contact Quest at 800-877-7484
• Reference Quest account number (on QPassport) or Specimen ID (on Quest paper form).

Drug Testing Fees

Express Labs charges $80 for DOT & Non-DOT drug test results and $125 for return-to-duty & follow-up drug test results (observed).

• Collection fees: Included at In-Network sites - Not included at Out-of-Network sites
• Invoices are due upon receipt: Members must maintain a valid credit card on file to keep company and drivers active

DOT Drug Testing Process

Collection → Laboratory Testing → MRO Review → Final Result

1. Collection Process

• Driver reports to a DOT certified collection site with the QPassport or the paper Quest form
• Collector verifies the driver’s ID and explains the steps
• Driver washes hands and removes outer clothing
• Split Urine Sample is collected:
  • Bottle A: 30 ml (for initial test)
  • Bottle B: 15 ml (for confirmation if needed)
• Sample temperature checked, sealed and labeled on the custody and control form (CCF)
• Sample shipped to Quest Diagnostics laboratory for testing

2. Laboratory Testing – Quest Diagnostics

• Initial Screening (Immunoassay)
  • If Negative the result is reported as negative to the MRO
  • If Positive the sample is sent for confirmation testing
• Confirmation Test (GC/MS) detects:
  • Marijuana (THC)
  • Cocaine
  • Opiates (codeine, morphine, heroin)
  • Amphetamines (methamphetamine, MDMA)
  • Phencyclidine (PCP)

3. Medical Review Officer (MRO) Review (licensed physician)

• Negative Results: The MRO reports the results as negative
• Non-Negative Results: MRO contacts the driver to confirm any valid prescriptions or medical explanations.
  • If the driver provides a valid medication prescription the results are reported as negative
  • If there is no medication prescription the results are reported as positive
  • DOT only allows the MRO to discuss prescription medications with the driver

Express Labs FMCSA Random Drug & Alcohol Consortium
Drug Testing | Updated 2026