Drug Testing Instructions

Order DOT Drug Tests online through your Express Labs Account or use the Quest Paper Forms we provide.  Once enrolled, only DOT Drug Tests ordered through Express Labs or completed using Express Labs Quest Paper Forms are accepted.

Order Drug Tests

1. Log in to your Express Labs Account and click Order Drug Tests
2. Select the Reason for Testing
3. Select a Collection Site then click Complete Order
4. Your QPassport is available immediately in Tests Ordered & QPassports on the left side of your Account and is also emailed within about 15 minutes
5. Bring the QPassport (printed or on your phone) with Photo ID to the Collection Site listed on the QPassport
6. Call the Collection Site to confirm they are open and accepting walk-ins

• Most Collection Sites will not begin a DOT collection within 3 hours of closing
• Drivers should only sign DOT forms that list the correct Employer/Company

Changing a Drug Test Order

• To change Driver information or the Collection Site, simply order a new test; Members are charged for Results, not unused QPassport orders

Using Quest Paper Forms

Use the Quest Paper Forms we provide at any Collection Site that collects DOT Drug Tests for Quest Diagnostics.

1. In-Network Collection Sites - Collection fees are included
2. Out-of-Network Collection Sites - Collection fees not included.  Search Google for "DOT Drug Testing"
3. Bring the Quest Paper Forms with Photo ID to the Collection Site
4. Call the Collection Site to confirm they are open and accepting walk-ins

• Most Collection Sites will not begin a DOT collection within 3 hours of closing

Drug Test Results

Drug Test Results are Emailed and Posted to your Express Labs Account.

• Most Negative Results are Reported within 48–72 hours after Quest Laboratory receives the Sample
• Weekend Collections: Samples collected over the weekend generally arrive at Quest Laboratory on Tuesday
• Non-Negative Results: May take 3–5 business days
• Delayed Results: Contact Quest Diagnostics at 800-877-7484 with your QPassport or Specimen ID

Cancelled Drug Tests

A Cancelled DOT Drug Test is not a positive or negative result.  The next step depends on the Reason for Cancellation.

• Cancelled Drug Test Instructions

Drug Testing Fees

Invoices are due upon receipt.  Members must maintain a valid credit card on file for Drug & Alcohol Testing charges and to Order Drug & Alcohol Tests.

• DOT & Non-DOT Drug Testing - $80
• Return-to-Duty & Follow-Up Drug Testing (Direct Observation) - $125
• Collection fees are included at In-Network Collection Sites only
• Paying Invoices Instructions

Drug Test Results from Other Companies

Once Enrolled, only DOT Drug Tests Ordered through Express Labs or completed using Express Labs Quest Paper Forms are accepted.

• The Result must list your Company as the Employer and Express Labs for MIS reporting.

Drug Testing Process

1. Specimen Collection

The DOT collection process begins at the Collection Site.

• Driver provides a urine specimen
• Collector checks the specimen and completes the Federal Custody & Control Form (CCF)
• Specimen is divided into two bottles:
  • Bottle A — Primary Specimen
  • Bottle B — Split Specimen
• Both bottles are sealed in the driver's presence
• Specimen is packaged and shipped to Quest Diagnostics

2. Laboratory Testing

Quest Diagnostics performs the required DOT laboratory testing.

• Laboratory verifies the specimen seals and CCF information
• Bottle A (Primary Specimen) is used for laboratory testing
• If the initial test is Negative, the result is sent to the Medical Review Officer (MRO)
• If the initial test is Non-Negative, the specimen undergoes confirmation testing
• Confirmed Non-Negative laboratory results are sent to the MRO for review

3. Medical Review Officer (MRO)

The MRO reviews the laboratory findings and determines the Final Verified Result.

• Reviews the laboratory findings and CCF documentation
• Negative results are verified and reported as Negative
• For a confirmed Non-Negative laboratory result, the MRO contacts the driver when required
• Driver has an opportunity to provide a legitimate medical explanation
• Only the MRO can review the driver's prescriptions and medical documentation
• Negative Results
• Positive Results

Express Labs FMCSA Random Drug & Alcohol Consortium
Drug Testing | Updated 2026