Drug Testing Instructions

Order DOT Drug Tests online or use the Quest Paper Forms we provide.  Once enrolled, only DOT Drug Tests ordered through Express Labs or completed using Express Labs Quest Paper Forms are accepted.

Order Drug Tests

1. Log in to your Express Labs account and click Order Drug Tests
2. Select the Reason for Testing
3. Select a Collection Site then click Complete Order
4. Check your email for the QPassport (within 1 hour)
5. Bring the QPassport (printed or on your phone) with Photo ID to the Collection Site
6. Call the Collection Site to confirm they are open and accepting walk-ins

• Most sites will not begin a DOT collection within 3 hours of closing
• Drivers should only sign DOT forms listing their employer/company name
• Members are charged for Results not QPassport orders

Using Quest Paper Forms

Use the Quest Paper Forms we provide at any site that collects DOT drug tests for Quest Diagnostics.

1. In-Network Collection Sites (collection fees included)
2. Out-of-Network Collection Sites (collection fees not included) - Search Google for "DOT Drug Testing"
3. Bring the Quest Paper Forms with Photo ID to the Collection Site
4. Call the Collection Site to confirm they are open and accepting walk-ins

• Most sites will not begin a DOT collection within 3 hours of closing

Drug Test Results

1. Emailed to the Designated Employer Representative (DER)
2. Uploaded to your Express Labs account
3. Available with Real-Time Test Tracking

• Most Negative Results are within 48-72 hours from when Quest Laboratory receives the Sample
• Weekend Collections: Samples arrive at Quest Laboratory on Tuesday
• Non-Negative Results: May take 3-5 business days
• Delayed Results:  Contact Quest Diagnostics at 800-877-7484 with your QPassport or Specimen ID

Drug Testing Fees

• DOT & Non-DOT Drug Tests: $80
• Return-to-Duty & Follow-Up Drug Tests (Observed): $125
• Collection fees included at In-Network sites only
• Invoices are due upon receipt
• A valid credit card is required to maintain active accounts

Drug Testing Process

1. Specimen Collection

The DOT collection process begins at the Collection Site.

• Driver provides a urine specimen
• Collector checks the specimen and completes the Federal Custody & Control Form (CCF)
• Specimen is divided into two bottles:
  • Bottle A — Primary Specimen
  • Bottle B — Split Specimen
• Both bottles are sealed in the driver's presence
• Specimen is packaged and shipped to Quest Diagnostics

Collection Site → Laboratory Testing

2. Laboratory Testing

Quest Diagnostics performs the required DOT laboratory testing.

• Laboratory verifies the specimen seals and CCF information
• Bottle A (Primary Specimen) is used for laboratory testing
• If the initial test is Negative, the result is sent to the Medical Review Officer (MRO)
• If the initial test is Non-Negative, the specimen undergoes confirmation testing
• Confirmed Non-Negative laboratory results are sent to the MRO for review

Laboratory Testing → MRO Review

3. Medical Review Officer (MRO)

The MRO reviews the laboratory findings and determines the Final Verified Result.

• Reviews the laboratory findings and CCF documentation
• Negative results are verified and reported as Negative
• For a confirmed Non-Negative laboratory result, the MRO contacts the driver when required
• Driver has an opportunity to provide a legitimate medical explanation
• Only the MRO can review the driver's prescriptions and medical documentation
• Negative Results
• Positive Results

Laboratory Testing → MRO Review → Final Results

Express Labs FMCSA Random Drug & Alcohol Consortium
Drug Testing | Updated 2026