Positive Results & Refusal-to-Test Instructions
Positive Results Instructions
Employers must remove the driver from safety-sensitive duties immediately and provide a Substance Abuse Professional (SAP) referral.
SAP Referral (Substance Abuse Professional)
- Provide the driver with a SAP referral immediately
- Document that the driver was given a SAP referral
- Find an SAP Referral - View SAPs
Clearinghouse Reporting
- Positive drug test results are reported to the Clearinghouse by the Express Labs MRO
Can Drivers be Re-Tested?
- No. DOT does not allow re-testing after a positive drug test result
Split Specimen Testing (Contested Results)
When a driver requests split specimen testing through the MRO, the process begins immediately. Sample B is sent to a second SAMHSA-certified laboratory for additional testing.
- Drivers have the right to request split specimen testing
- Employers may not delay, cancel, or interfere with the process
- Drivers must contact the MRO within the required timeframe
- Express Labs charges $200 per positive drug test for split specimen shipping and testing
Refusal-to-Test Instructions
It's the employer's responsibility — not the collector's — to decide whether a driver's conduct counts as a refusal to test - based on the collector's notes plus any information the collector or driver provides.
Employers must remove the driver from safety-sensitive duties immediately and provide a Substance Abuse Professional (SAP) referral. A refusal-to-test must be treated the same as a positive drug test. In the event of an Audit - you need to be able to show exactly why it was (or wasn't) a refusal.
Refusal-to-Test Includes:
- Failure to appear for testing
- Leaving the collection site before the test is completed
- Refusing to provide a specimen
- Tampering with a specimen
- DOT Policy for Refusal-to-Test - View Policy
- DOT Policy for "Get Tested Immediately" (page 19) - View Policy
SAP Referral (Substance Abuse Professional)
- Provide the driver with a SAP referral immediately
- Document that the driver was given a SAP referral
- Find an SAP Referral - View SAPs
Clearinghouse Reporting
Positive Alcohol Test Instructions
Employers must remove the driver from safety-sensitive duties immediately and provide a Substance Abuse Professional (SAP) referral.
- Clearinghouse Reporting - DOT requires employers to report positive alcohol tests to the Clearinghouse – View Instructions
Alcohol Test Results
- 0.04 or higher — Remove from duty and begin the SAP process
- 0.02–0.039 — Remove from duty for 24 hours
- Below 0.02 — Negative result
SAP Referral (Substance Abuse Professional)
- Provide the driver with a SAP referral immediately
- Document that the driver was given a SAP referral
- Find an SAP Referral - View SAPs
FMCSA Clearinghouse Reporting
Express Labs FMCSA Random Drug & Alcohol Consortium
Positive Results | Refusal-to-Test | Updated 2026