DOT Authority#

Positive Results & Refusal-to-Test Instructions


Employers must remove the driver from driving immediately and provide the driver with an SAP referral.  Drivers must complete the SAP process and Return-to-Duty testing before returning to driving.

  1. Remove the driver from duty immediately
  2. Provide the driver with an SAP referral
  • Express Labs MRO reports positive results to the FMCSA Clearinghouse
  • DOT requires employers to report refusals and positive alcohol tests to the Clearinghouse

SAP Referral (Substance Abuse Professional)

Find an SAP Referral - View SAPs

  • Document that the driver was given a SAP referral

Can Drivers be Re-Tested?

No. DOT does not allow re-testing of a positive result


Split Specimen Testing (Contested Results)

When a driver requests split specimen testing through the MRO, the process begins immediately.  Sample B is sent to a second SAMHSA-certified laboratory for additional testing.

Important Information

  • Drivers have the right under DOT regulations to request split specimen testing
  • Employers may not delay, cancel, or interfere with the process for any reason
  • Only the driver may withdraw the request, and only before the process begins
  • Drivers must contact the MRO directly at 800-526-9341 to request or withdraw testing
  • Express Labs charges a $200 hazmat shipping fee for FedEx shipment of Sample B
  • The original positive result remains valid unless the split specimen test determines otherwise

Refusal-to-Test Instructions

DOT requires employers to determine whether a test is a Refusal-to-Test based on the collector's notes and conversations with the collector and driver.  A refusal-to-test is treated the same as a positive result.  Employers must remove the driver from driving immediately and provide the driver with an SAP referral.  Drivers must complete the SAP process and Return-to-Duty testing before returning to driving.

  1. Remove the driver from duty immediately
  2. Provide the driver with an SAP referral
  3. Report the refusal to the FMCSA Clearinghouse within 3 business days

Refusal-to-Test may include:

  • Failure to appear for testing
  • Leaving the collection site before the test is completed
  • Refusing to provide a specimen
  • Tampering with a specimen

SAP Referral (Substance Abuse Professional)

Find an SAP Referral - View SAPs

  • Document that the driver was given a SAP referral

FMCSA Clearinghouse Reporting – View Instructions

Employers must report refusals and positive alcohol tests to the Clearinghouse

  • Express Labs is required to report owner-operators to the Clearinghouse for refusal-to-test and positive alcohol tests

DOT Policy for Refusal-to-Test

Drivers who do not complete testing immediately after notification are considered to have refused-to-test, which carries the same consequences as a positive result.

  • DOT Policy for Refusal-to-Test - View Policy
  • DOT Policy for "Get Tested Immediately" (page 19) - View Policy

Positive Alcohol Test Instructions

If a driver has a positive alcohol test (0.04 or higher):

  1. Remove the driver from duty immediately
  2. Provide a SAP referral
  3. Report the result to the FMCSA Clearinghouse

Alcohol Test Results

  • 0.04 or higher (Positive): Remove from duty + SAP process
  • 0.02–0.039 (Not Positive): Remove from duty for 24 hours
  • Below 0.02: Negative

Compliance Notice

Positive tests and refusals are permanent DOT violations recorded in the FMCSA Clearinghouse and must be completed through the SAP process before returning to safety-sensitive duties.


Express Labs FMCSA Random Drug & Alcohol Consortium
Positive Results | Refusal-to-Test | Updated 2026